Veterinary World

N. Mohammad Sharif ¹, B. Sreedevi ¹, R. K. Chaitanya ¹, D. Sreenivasulu ¹

Affiliations
1 Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, IN

Abstract

Aim: The aim of this study was to characterize beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh.
Materials and Methods: A total of 136 rectal swabs were collected from healthy (92) and diarrheic (44) dogs, bacteriological cultured for Klebsiella and Enterobacter growth and screened for beta-lactamase antimicrobial resistance phenotypically by disc diffusion method and genotypically by polymerase chain reaction targeting bla_TEM, bla_SHV, bla_OXA, bla_CTX-M Group 1, 2, bla_AmpC, bla_ACC, and bla_MOX genes.
Results: A total of 33 Klebsiella and 29 Enterobacter isolates were recovered. Phenotypic beta-lactamase resistance was detected in 66.6% and 25% of Klebsiella and Enterobacter isolates, respectively, from healthy dogs and 66.6% and 60% of Klebsiella and Enterobacter isolates, respectively, from diarrheic dogs. Overall, incidence of extended-spectrum beta-lactamase (ESBL) phenotype was found to be 21.2% (7/33) in Klebsiella isolates, whereas none of the Enterobacter isolates exhibited ESBL phenotype. Predominant beta-lactamase genes detected in Klebsiella species include bla_SHV (84.8%), followed by bla_TEM (33.3%), bla_CTX-M Group 1 (15.1%), and bla_OXA (6.1%) gene. Predominant beta-lactamase genes detected in Enterobacter species include bla_SHV (48.2%), followed by bla_TEM (24.1%), bla_AmpC (13.7%), and bla_OXA (10.3%) gene.
Conclusion: The present study highlighted alarming beta-lactamase resistance in Klebsiella and Enterobacter species of canine origin in India with due emphasis as indicators of antimicrobial resistance.

Keywords

Beta-Lactamase Resistance, Dogs, Enterobacter, Extended-Spectrum Beta-Lactamase, Klebsiella.

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